The study determined predictive roles of workplace relationship and organizational culture on organizational commitment among college of Education Staff of Nsugbe, Anambra State. The target population consisted of academic and non-academic staff. A total number of 227 staff served as participants for the study. The participants were selected through non-probability sample (incident sampling techniques). The gender data showed that 132(58.1%) were females and 95(41.9%) were males. The participants’ age ranged from 21 to 56 with mean age of 34.86 and standard deviation of 11.99. Three instruments were adopted for the study: Organizational Commitment Scale (OCS), Workplace Relationship Scale and Denison Organizational Culture Scale. The study used correlational design for the study and Multiple Linear Regression Statistics as appropriate statistics. The study revealed that workplace relationship (interpersonal regard, mutual acceptance, mutual trust and workplace harmony) and organisational culture (involvement, consistency, adaptability and mission) accounted for 80.2% of the organisational commitment, with R2 = .802, adjusted R2=.795, (F8, 218) = 110.56, p<.05 shows that the overall model has significant contribution to organisational commitment at 80.2%. Interpersonal regard of workplace relationship at (F8, 218) β= -.29, t = -3.75, and mutual trust of workplace relationship at (F8, 218) β= -.72, t = -14.20, had negative predictive effect on organizational commitment at p<.05, while mutual acceptance of workplace relationship at (F8, 218) β= 10.44, t = 1.04 and workplace harmony of workplace relationship a t(F8, 218) β= 7.71, t = 1.73 had positive prediction on organizational commitment at p<.05. Involvement of organizational culture at (F8, 218) β= -.61, t = -10.65, consistency of organizational culture at (F8, 218) β= -.89, t = -4.25, and adaptability of organizational culture at (F8, 218) β= -.30, t = -3.24 had negative predictive effect on organizational commitment at p<.05, whereas mission of organizational culture at (F8, 218) β= .46, t = 9.82 had positive prediction on organizational commitment at p<.05. The findings suggested that management should inculcate the culture of encouraging employee participation in decision-making processes and provide opportunities for growth and development.